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RNAzol®RTisthemosteffectivereagentforisolationoftotalRNAandsmallRNAfromsamplesofhuman,animal,plant,bacterialandviralorigin.Thispatentedreagent(1)provideshigheryieldandqualityofisolatedRNAthanpreviousreagentsbasedonthesingle-stepmethod.RNAzol®RTisolatespureandundegradedRNAthatisreadyforRT-PCRwithoutDNasetreatment.(2)

• Nochloroform-inducedphaseseparationisnecessarytoobtainpureRNA.JustaddwatertoremoveDNA,proteins,polysaccharidesandothercontaminants.
• Theisolationprocedurecanbecompletedinlessthanonehourandisperformedatroomtemperature,includingallcentrifugationsteps.
• RNAzol®RTisolatestotalRNA,orlargeRNAandsmallRNAinseparatefractions.ThelargeRNAfractioncontainsrRNAandmRNA.ThesmallRNAfractioncontainstRNA,smallRNAandmicroRNAdownto10bases.
• TheisolatedRNAisreadyforRT-PCR,qRT-PCR,microarrays,polyA+selection,northernblotting,RNaseprotectionassayandothermolecularBIOLOGyapplications.
• Duetotheremovalofimpurities,theRNApelletsaresmallerandsolubilizemoreeasilythanpelletsobtainedfromprevioussingle-stepreagents.
• Inaddition,RNAzol®RTallowsforthesimultaneousisolationofRNAandDNA.

RNAzol®RTisusedtoisolateRNAfromtissues,cells,liquidsamplesorblood.Onemilliliterissufficienttoprocessupto100mgtissueyielding50–700μgoflargeRNA(>200bases)and8–120μgofsmallRNA(200–10bases).

References

1. USpatents7,794,932and8,367,817.Internationalpatents.
2. Chomczynski,P.etal.RNAzolRT:Anewsingle-stepmethodforisolationofRNA.NatureMethods.December,2010.