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分离和分析RNA和DNA的产品
℡ 4000-520-616
℡ 4000-520-616
mrcgene/TRI Reagent® RT / TRI Reagent® RT-Liquid Samples|||TRI Reagent® RT (RT 111)/RT 111-200-ml
产品编号:RT111-200-ml
市  场 价:¥5480.00
场      地:美国(厂家直采)
产品分类: 分子类>基因编辑>RNA干扰>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$274.00
品      牌: mrcgene
公      司:Molecular Research Center
公司分类:
mrcgene/TRI Reagent® RT / TRI Reagent® RT-Liquid Samples|||TRI Reagent® RT (RT 111)/RT 111-200-ml
商品介绍

TRI Reagent® RT is an upgraded version of the single-step method of RNA isolation. TRI Reagent® RT is based on the phase separation method using phenol and guanidine thiocyanate. This patent-pending reagent substantially improves the quality of isolated RNA. Unlike the previous single-step methods, TRI Reagent® RT isolates RNA that is free of DNA contamination. The purity of RNA was tested by a 35-cycle PCR. Thus, no DNase treatment is necessary to use the isolated RNA in RT-PCR. Also, the isolated RNA solubilizes more quickly and easily as compared with the older single-step methods.

TRI Reagent® RT provides a reliable, cost-effective and efficient method of RNA isolation. It is a comprehensive reagent available for analysis of gene expression. TRI Reagent® RT isolates RNA from a wide variety of samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent® RT also can be used to simultaneously isolate RNA, DNA and proteins from the same sample. The RNA isolation is completed in less than 1 hour. In addition to total RNA, TRI Reagent® RT can be used to isolate high and low molecular weight RNA as separate fractions.

TRI Reagent® RT (RT 111)

$83.00$646.00

Full Protocol (PDF) |SDS (PDF) | View Product on U.S. Price List

TRI Reagent® RT is used for RNA isolation from tissues, pelleted cells and cells grown in monolayer. Fifty milliliters is sufficient to process 50 samples, each containing 50 mg of tissue. Expected yields range from 50 – 700 μg of RNA per sample, depending upon the tissue source.

SKU: RT 111Category: RNA Isolation

References

  1. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate phenol-chloroform extraction. Anal. Biochem. 162:156-159, 1987.
  2. Chomczynski, P., US patents 4,843,155 and 5,346,994.
  3. Chomczynski, P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15:532-537, 1993.
品牌介绍

由Piotr Chomczynski于1989年成立的Molecular Research Center,Inc.开发和制造用于RNA和DNA分离和分析的创新产品。它是一家“由科学家为科学家经营的公司”,我们一直在致力于分子生物学技术的发展和改进。

从用于RNA分离的单步方法开始,我们致力于简化核酸分离方案,同时提高了回收率和样品纯度。该方法已成为过去25年中被引用次数最多的科学出版物。

我们获得专利的RNA和DNA提取技术在全球范围内被公认为具有最佳性能,可靠性和成本效益。


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